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TEM images of Bacillus sp. S9 ( A ) and Enterobacter sp. Z11 ( B ) after 7 days of incubation with 50 mg L⁻¹ Cr(VI) under anaerobic conditions. Black arrows indicate electron-dense intracellular deposits corresponding to Cr accumulation. Panels ( C ) and ( D ) <t>show</t> <t>STEM-EDX</t> of thin cell sections from Bacillus sp. S9 and Enterobacter sp. Z11, respectively. The insert images display the analyzed regions (highlighted by boxes). The EDX spectra confirm the presence of Cr, with characteristic Cr Lα and Cr Kβ peaks detected alongside carbon (C) and oxygen (O) signals from the cellular matrix. Quantitative elemental composition (expressed as weight%) is shown in the embedded tables, indicating significant Cr enrichment within the cells. Scale bars: 100 nm ( A , B ) and 1 μm ( C , D )
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TEM images of Bacillus sp. S9 ( A ) and Enterobacter sp. Z11 ( B ) after 7 days of incubation with 50 mg L⁻¹ Cr(VI) under anaerobic conditions. Black arrows indicate electron-dense intracellular deposits corresponding to Cr accumulation. Panels ( C ) and ( D ) <t>show</t> <t>STEM-EDX</t> of thin cell sections from Bacillus sp. S9 and Enterobacter sp. Z11, respectively. The insert images display the analyzed regions (highlighted by boxes). The EDX spectra confirm the presence of Cr, with characteristic Cr Lα and Cr Kβ peaks detected alongside carbon (C) and oxygen (O) signals from the cellular matrix. Quantitative elemental composition (expressed as weight%) is shown in the embedded tables, indicating significant Cr enrichment within the cells. Scale bars: 100 nm ( A , B ) and 1 μm ( C , D )
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TEM images of Bacillus sp. S9 ( A ) and Enterobacter sp. Z11 ( B ) after 7 days of incubation with 50 mg L⁻¹ Cr(VI) under anaerobic conditions. Black arrows indicate electron-dense intracellular deposits corresponding to Cr accumulation. Panels ( C ) and ( D ) <t>show</t> <t>STEM-EDX</t> of thin cell sections from Bacillus sp. S9 and Enterobacter sp. Z11, respectively. The insert images display the analyzed regions (highlighted by boxes). The EDX spectra confirm the presence of Cr, with characteristic Cr Lα and Cr Kβ peaks detected alongside carbon (C) and oxygen (O) signals from the cellular matrix. Quantitative elemental composition (expressed as weight%) is shown in the embedded tables, indicating significant Cr enrichment within the cells. Scale bars: 100 nm ( A , B ) and 1 μm ( C , D )
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TEM images of Bacillus sp. S9 ( A ) and Enterobacter sp. Z11 ( B ) after 7 days of incubation with 50 mg L⁻¹ Cr(VI) under anaerobic conditions. Black arrows indicate electron-dense intracellular deposits corresponding to Cr accumulation. Panels ( C ) and ( D ) <t>show</t> <t>STEM-EDX</t> of thin cell sections from Bacillus sp. S9 and Enterobacter sp. Z11, respectively. The insert images display the analyzed regions (highlighted by boxes). The EDX spectra confirm the presence of Cr, with characteristic Cr Lα and Cr Kβ peaks detected alongside carbon (C) and oxygen (O) signals from the cellular matrix. Quantitative elemental composition (expressed as weight%) is shown in the embedded tables, indicating significant Cr enrichment within the cells. Scale bars: 100 nm ( A , B ) and 1 μm ( C , D )
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TEM images of Bacillus sp. S9 ( A ) and Enterobacter sp. Z11 ( B ) after 7 days of incubation with 50 mg L⁻¹ Cr(VI) under anaerobic conditions. Black arrows indicate electron-dense intracellular deposits corresponding to Cr accumulation. Panels ( C ) and ( D ) show STEM-EDX of thin cell sections from Bacillus sp. S9 and Enterobacter sp. Z11, respectively. The insert images display the analyzed regions (highlighted by boxes). The EDX spectra confirm the presence of Cr, with characteristic Cr Lα and Cr Kβ peaks detected alongside carbon (C) and oxygen (O) signals from the cellular matrix. Quantitative elemental composition (expressed as weight%) is shown in the embedded tables, indicating significant Cr enrichment within the cells. Scale bars: 100 nm ( A , B ) and 1 μm ( C , D )

Journal: 3 Biotech

Article Title: Removal of hexavalent chromium by facultative anaerobic strains Bacillus sp. S9 and Enterobacter sp. Z11 from a mining site microbial mat

doi: 10.1007/s13205-026-04841-9

Figure Lengend Snippet: TEM images of Bacillus sp. S9 ( A ) and Enterobacter sp. Z11 ( B ) after 7 days of incubation with 50 mg L⁻¹ Cr(VI) under anaerobic conditions. Black arrows indicate electron-dense intracellular deposits corresponding to Cr accumulation. Panels ( C ) and ( D ) show STEM-EDX of thin cell sections from Bacillus sp. S9 and Enterobacter sp. Z11, respectively. The insert images display the analyzed regions (highlighted by boxes). The EDX spectra confirm the presence of Cr, with characteristic Cr Lα and Cr Kβ peaks detected alongside carbon (C) and oxygen (O) signals from the cellular matrix. Quantitative elemental composition (expressed as weight%) is shown in the embedded tables, indicating significant Cr enrichment within the cells. Scale bars: 100 nm ( A , B ) and 1 μm ( C , D )

Article Snippet: To determine the intracellular accumulation of Cr, Transmission Electron Microscopy (TEM) (JEOL, TEM 1200EX, Japan) and Scanning Transmission Electron Microscopy with Energy-Dispersive X-ray spectroscopy (STEM-EDX) (JEOL, JEM 2100 F, Japan) were employed.

Techniques: Incubation